Identification and Characterisation of Stripe Rust Resistance Genes Yr66 and Yr67 in Wheat Cultivar VL Gehun 892

Wheat cultivar VL Gehun 892 has shown a high level of resistance against Australian Puccinia striiformis f. sp. tritici (Pst) pathotypes. In this study, it was crossed with Westonia, susceptible wheat cultivar, and digenic segregation observed in the derived population Pst pathotype 134 E16A+Yr17+Yr27+. Single-gene recombinant inbred line (RIL) populations were developed from F3 families (VL 892/Westonia#1 VLGehun 892/Westonia#4) that showed monogenic segregations two distinct phenotypes. E16A+Yr17+Yr27+ confirmed both F6 RIL populations. Bulked segregant analysis using 90K Infinium SNP array placed YrVL1 short arm chromosome 3D YrVL2 long 7B. Kompetitive allele specific polymerase chain reaction (KASP) assays for SNPs linked mapped onto respective KASP_48179 (0.6 cM proximal) KASP_18087 (2.1 distal) flanked YrVL1, whereas between KASP_37096 (1.2 KASP_2239 (3.6 distal). Based on their pathotypic specificities, map locations, stages expression, demonstrated to be unique loci named Yr66 Yr67, respectively. Markers these genes more than 85% polymorphism when tested set 89 cultivars hence could used marker-assisted selection breeding programs, following checks parental polymorphisms.